Part:BBa_K238000

YcgF/E regulated promoter region

ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the reporssor from the promoter region. see reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)

the actuall promotor region was later identified with the help of Regine Hengge ang her lab. to see the part for the exact region check out part BBa_K238013.

making this part was not continued.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 178
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 178
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 178
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 178
  
• 1000
  COMPATIBLE WITH RFC[1000]